Coding
Part:BBa_M10044:Design
Designed by: Sam Ng Group: Berkeley BioE140L - S09 (2009-05-10)
{˂espP(beta)!} autotransporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 685
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is a BglBricks (BBb). No prepro; Begins with amino acid 852 and extends to the stop codon. Part was inserted into pBca9495KC-Bca1144#5.
Source
Bglbricking of espP(beta) PCR osn0001/osn0002 on Escherichia coli O157:H7 str. Sakai (1381 bp, EcoRI/BamHI) Sub into pBca9495KC-Bca1144 (EcoRI/BamHI, 2974+910, L) Product is pBca9495KC-M10044 {<espP(beta)!} ------------------------------------- Osn0001-F Forward Bglbricking of espP of Escherichia coli O157:H7 str. Sakai ccagtGAATTCatgAGATCTgccccgtcagcatctgccac Osn0002-R Reverse Bglbricking of espP of Escherichia coli O157:H7 str. Sakai gcagtGGATCCtcagaacgagtaacggaaattag
References
PMID: 16262782