Coding

Part:BBa_M10044:Design

Designed by: Sam Ng   Group: Berkeley BioE140L - S09   (2009-05-10)

{˂espP(beta)!} autotransporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 685
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a BglBricks (BBb). No prepro; Begins with amino acid 852 and extends to the stop codon. Part was inserted into pBca9495KC-Bca1144#5.


Source

Bglbricking of espP(beta)
PCR osn0001/osn0002 on Escherichia coli O157:H7 str. Sakai           (1381 bp, EcoRI/BamHI)
Sub into pBca9495KC-Bca1144                                          (EcoRI/BamHI, 2974+910, L)
Product is pBca9495KC-M10044	{<espP(beta)!}
-------------------------------------
Osn0001-F  Forward Bglbricking of espP of Escherichia coli O157:H7 str. Sakai    ccagtGAATTCatgAGATCTgccccgtcagcatctgccac
Osn0002-R  Reverse Bglbricking of espP of Escherichia coli O157:H7 str. Sakai    gcagtGGATCCtcagaacgagtaacggaaattag

References

PMID: 16262782